CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression.
basic_science · Level V
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- Record sourced from PubMed, PMID 18438402.
- Also identified by DOI 10.1038/nbt1399.
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Abstract
Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflammatory response, whereas CpG-free pDNA vectors do not. Using a CpG-free pDNA expression vector, we achieved sustained (>or=56 d) in vivo transgene expression in the absence of lung inflammation.
Medical subject headings
- CpG Islands
- Gene Targeting
- Genetic Therapy
- Inflammation
- Lung
- Plasmids