Single bead affinity detection (SINBAD) for the analysis of protein-protein interactions.
basic_science · Level V
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- Record sourced from PubMed, PMID 18446240.
- Also identified by DOI 10.1371/journal.pone.0002061 and PMC identifier PMC1796766.
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Abstract
We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on single affinity chromatography beads by fluorescence microscopy. The use of QDs for single molecule detection allows the simultaneous analysis of multiple protein-protein binding events and reduces the amount of time and material needed to perform a pull-down experiment.