Protein-membrane interaction probed by single plasmonic nanoparticles.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 18459744.
- Also identified by DOI 10.1021/nl080805l.
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Abstract
We present a nanosized and addressable sensor platform based on membrane coated plasmonic particles and show unequivocally the covering with lipid bilayers as well as the subsequent detection of streptavidin binding to biotinylated lipids. The binding is detected on membrane covered gold nanorods by monitoring the spectral shift by fast single particle spectroscopy (fastSPS) on many particles in parallel. Our approach allows for local analysis of protein interaction with biological membranes as a function of the lateral composition of phase separated membranes.
Medical subject headings
- Cell Membrane
- Membrane Proteins
- Molecular Probe Techniques
- Nanoparticles
- Protein Interaction Mapping
- Surface Plasmon Resonance