Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 18469823.
- Also identified by DOI 10.1038/nmeth.1211.
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Abstract
Imaging volumes as thick as whole cells at three-dimensional (3D) super-resolution is required to reveal unknown features of cellular organization. We report a light microscope that generates images with translationally invariant 30 x 30 x 75 nm resolution over a depth of several micrometers. This method, named biplane (BP) FPALM, combines a double-plane detection scheme with fluorescence photoactivation localization microscopy (FPALM) enabling 3D sub-diffraction resolution without compromising speed or sensitivity.
Medical subject headings
- Biophysics
- Imaging, Three-Dimensional
- Microscopy, Fluorescence