Next-generation high-density self-assembling functional protein arrays.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 18469824.
- Also identified by DOI 10.1038/nmeth.1210 and PMC identifier 3070491.
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Abstract
We developed a high-density self-assembling protein microarray, based on the nucleic acid programmable protein array (NAPPA) concept, to display thousands of proteins that are produced and captured in situ from immobilized cDNA templates. We arrayed up to 1,000 unique human cDNAs and obtained high yields of protein expression and capture with minimal variation and good reproducibility. This method will enable various experimental approaches to study protein function in high throughput.
Medical subject headings
- Gene Expression Profiling
- Protein Array Analysis
- Proteomics