Oligo-microarray based on oligonucleotide immobilization on glass surface modified with activated acrylic acid-co-acrylamide copolymer.

Abstract

Oligo-microarray can be fabricated on modified surface of glass slides involving immobilization of oligonucleotides. In this study, glass slides were modified with acrylic acid-co-acrylamide copolymer and EDC (1-ethyl-3(3-dimethylaminopropyl)-carbodiimide)/NHS (N-hydroxysuccinimide) by covalent linkage method, and covalent immobilization of unmodified oligonucleotide on the glass surface was achieved. The platform with binding of stable and sensitive oligonucleotides was prepared for oligo-microarray fabrication. An optimum concentration of 0.5 g/L was used for spotting. The lengths of 26 to 70 mer oligonucleotides were immobilized on the surface efficiently. The spots were approximately 160 nm in diameter and their mean value of fluorescent intensity was measured in the range of 3.2 x 10(4) to 7.3 x 10(4) after hybridization. The modified glass surface was scanned by SEM and the dendritic structure was observed. Results showed that the process of preparation of the modified glass surface was simple and cost effective, and the modified surface can be used for the oligo-microarray fabrication and also attachment of protein, PNA etc.