Nanofluidic biosensing for beta-amyloid detection using surface enhanced Raman spectroscopy.

Chou, I-Hsien; Benford, Melodie; Beier, Hope T; Coté, Gerard L; Wang, Miao; Jing, Nan; Kameoka, Jun; Good, Theresa A · Nano Lett · 2008

basic_science · Level V

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Abstract

Trace detection of the conformational transition of beta-amyloid peptide (Abeta) from a predominantly alpha-helical structure to beta-sheet could have a large impact in understanding and diagnosing Alzheimer's disease. We demonstrate how a novel nanofluidic biosensor using a controlled, reproducible surface enhanced Raman spectroscopy active site was developed to observe Abeta in different conformational states during the Abeta self-assembly process as well as to distinguish Abeta from confounder proteins commonly found in cerebral spinal fluid.

Medical subject headings