Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy.

Neuman, Keir C; Nagy, Attila · Nat Methods · 2008

review · Level V

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Abstract

Single-molecule force spectroscopy has emerged as a powerful tool to investigate the forces and motions associated with biological molecules and enzymatic activity. The most common force spectroscopy techniques are optical tweezers, magnetic tweezers and atomic force microscopy. Here we describe these techniques and illustrate them with examples highlighting current capabilities and limitations.

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