Lifeact: a versatile marker to visualize F-actin.
basic_science · Level V
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- Record sourced from PubMed, PMID 18536722.
- Also identified by DOI 10.1038/nmeth.1220 and PMC identifier 2814344.
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Abstract
Live imaging of the actin cytoskeleton is crucial for the study of many fundamental biological processes, but current approaches to visualize actin have several limitations. Here we describe Lifeact, a 17-amino-acid peptide, which stained filamentous actin (F-actin) structures in eukaryotic cells and tissues. Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells.
Medical subject headings
- Actins
- Coloring Agents
- Peptides
- Staining and Labeling