Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 18568017.
- Also identified by DOI 10.1038/nbt1418 and PMC identifier 6334647.
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Abstract
Reprogramming of mouse and human somatic cells can be achieved by ectopic expression of transcription factors, but with low efficiencies. We report that DNA methyltransferase and histone deacetylase (HDAC) inhibitors improve reprogramming efficiency. In particular, valproic acid (VPA), an HDAC inhibitor, improves reprogramming efficiency by more than 100-fold, using Oct4-GFP as a reporter. VPA also enables efficient induction of pluripotent stem cells without introduction of the oncogene c-Myc.
Medical subject headings
- Cell Differentiation
- DNA (Cytosine-5-)-Methyltransferases
- Histone Deacetylase Inhibitors
- Stem Cells
- Valproic Acid