Alta-Cyclic: a self-optimizing base caller for next-generation sequencing.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 18604217.
- Also identified by DOI 10.1038/nmeth.1230 and PMC identifier 2978646.
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Abstract
Next-generation sequencing is limited to short read lengths and by high error rates. We systematically analyzed sources of noise in the Illumina Genome Analyzer that contribute to these high error rates and developed a base caller, Alta-Cyclic, that uses machine learning to compensate for noise factors. Alta-Cyclic substantially improved the number of accurate reads for sequencing runs up to 78 bases and reduced systematic biases, facilitating confident identification of sequence variants.
Medical subject headings
- Sequence Analysis, DNA
- Software