Improving membrane voltage measurements using FRET with new fluorescent proteins.
basic_science · Level V
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- Record sourced from PubMed, PMID 18622396.
- Also identified by DOI 10.1038/nmeth.1235.
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Abstract
We used two new coral fluorescent proteins as fluorescence resonance energy transfer (FRET) donor and acceptor to develop a voltage sensor, named Mermaid, that displays approximately 40% changes in emission ratio per 100 mV, allowing for direct visualization of electrical activities in cultured excitable cells. Notably, Mermaid has fast on-off kinetics at warm (approximately 33 degrees C) temperatures and can report voltage spikes comparable to action potentials.
Medical subject headings
- Cell Membrane
- Fluorescence Resonance Energy Transfer
- Luminescent Proteins
- Membrane Potentials