On-site manipulation of single chromosomal DNA molecules by using optically driven microstructures.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 18651069.
- Also identified by DOI 10.1039/b803753a.
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Abstract
We report a novel method for manipulation of single giant DNA molecules under a video microscope. Using optically driven microstructures, we manipulated chromosomal DNA of length in the order of millimetres, extended by electroosmotic flow without DNA breakage in aqueous solution: we picked up DNA, using microfabricated hooks and wound it around microfabricated bobbins.
Medical subject headings
- Chromosomes
- DNA, Fungal
- Microscopy, Electron, Scanning