Caenorhabditis elegans mutant allele identification by whole-genome sequencing.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 18677319.
- Also identified by DOI 10.1038/nmeth.1249 and PMC identifier 2574580.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Identification of the molecular lesion in Caenorhabditis elegans mutants isolated through forward genetic screens usually involves time-consuming genetic mapping. We used Illumina deep sequencing technology to sequence a complete, mutant C. elegans genome and thus pinpointed a single-nucleotide mutation in the genome that affects a neuronal cell fate decision. This constitutes a proof-of-principle for using whole-genome sequencing to analyze C. elegans mutants.
Medical subject headings
- Alleles
- Caenorhabditis elegans
- Genome, Helminth
- Mutation