Expression of the dengue virus type 2 NS5 protein in a CD4(+) T cell line inhibits HIV replication.

McLinden, James H; Stapleton, Jack T; Chang, Qing; Xiang, Jinhua · J Infect Dis · 2008

basic_science · Level V

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Abstract

Human immunodeficiency virus (HIV) load is suppressed during dengue virus infection. The NS5A phosphoprotein of GB virus C (a related flavivirus) inhibits HIV replication in vitro. To determine whether the dengue virus NS5 protein inhibits HIV replication, CD4(+) T cell lines expressing this protein were generated. HIV replication in dengue virus NS5-expressing cells decreased by >90% compared with control cells (P < .01), and this was mediated in part by decreased HIV coreceptor (CXCR4) expression and increased production of SDF-1. Thus, the dengue virus NS5 protein inhibits HIV replication in vitro, potentially explaining the reduction in HIV load observed during acute dengue virus infection.

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