Automated screening for mutants affecting dopaminergic-neuron specification in C. elegans.
basic_science · Level V
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- Record sourced from PubMed, PMID 18758453.
- Also identified by DOI 10.1038/nmeth.1250 and PMC identifier 2693092.
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Abstract
We describe an automated method to isolate mutant Caenorhabditis elegans that do not appropriately execute cellular differentiation programs. We used a fluorescence-activated sorting mechanism implemented in the COPAS Biosort machine to isolate mutants with subtle alterations in the cellular specificity of GFP expression. This methodology is considerably more efficient than comparable manual screens and enabled us to isolate mutants in which dopamine neurons do not differentiate appropriately.
Medical subject headings
- Caenorhabditis elegans
- Dopamine
- Mutation
- Neurons