Enhanced proliferation of human umbilical vein endothelial cells and differentiation of 3T3-L1 adipocytes in coculture.

Abstract

The interactions between adipocytes and endothelial cells in adipose tissue development are poorly understood. In this study, we characterized the growth and differentiation of 3T3-L1 preadipocytes and human umbilical vein endothelial cells (HUVECs) in planar and collagen gel cocultures. In planar coculture, preadipocyte proliferation was up to three times as great as in the control culture with only preadipocytes, where the increase was proportional to the HUVEC fraction in the seeding mixture. In the collagen gel coculture, triglyceride (TG) content (per adipocyte) was up to 3.4 times as much as in the control with only adipocytes. This effect depended on the total density and composition of the seeding mixture, with the largest increase observed at the highest density (2 x 10(6) cells/mL collagen) and preadipocyte:HUVEC ratio (90:10) tested in this study. Immunostaining showed that the collagen gel coculture also supported the elongation of endothelial cells. Blockade of vascular endothelial growth factor receptor 2 (VEGFR2) abolished the adipogenesis- and neovascularization-related effects of the coculture. Taken together, our results indicate that endothelial cell-mediated enhancement of adipocyte differentiation requires the activation of VEGFR2.