Super-resolution imaging in live Caulobacter crescentus cells using photoswitchable EYFP.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 18794860.
- Also identified by DOI 10.1038/nmeth.1258 and PMC identifier 2655310.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
The commonly used, monomeric EYFP enabled imaging of intracellular protein structures beyond the optical resolution limit ('super-resolution' imaging) in living cells. By combining photoinduced activation of single EYFP fusions and time-lapse imaging, we obtained sub-40 nm resolution images of the filamentous superstructure of the bacterial actin protein MreB in live Caulobacter crescentus cells. These studies demonstrated that EYFP is a useful emitter for in vivo super-resolution imaging.
Medical subject headings
- Caulobacter crescentus
- Luminescent Proteins
- Photosensitizing Agents
- Spectrometry, Fluorescence