Fluorescence nanoscopy by ground-state depletion and single-molecule return.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 18794861.
- Also identified by DOI 10.1038/nmeth.1257.
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Abstract
We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left or those that spontaneously returned to the ground state. Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine- and fluorescent protein-labeled (living) samples, proving a simple yet powerful super-resolution approach.
Medical subject headings
- Microscopy, Fluorescence
- Spectrometry, Fluorescence