Imaging individual mRNA molecules using multiple singly labeled probes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 18806792.
- Also identified by DOI 10.1038/nmeth.1253 and PMC identifier 3126653.
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Abstract
We describe a method for imaging individual mRNA molecules in fixed cells by probing each mRNA species with 48 or more short, singly labeled oligonucleotide probes. This makes each mRNA molecule visible as a computationally identifiable fluorescent spot by fluorescence microscopy. We demonstrate simultaneous detection of three mRNA species in single cells and mRNA detection in yeast, nematodes, fruit fly wing discs, and mammalian cell lines and neurons.
Medical subject headings
- Molecular Probes
- RNA, Messenger