A microfluidic flow injection system for DNA assay with fluids driven by an on-chip integrated pump based on capillary and evaporation effects.
basic_science · Level V
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- Record sourced from PubMed, PMID 18813387.
- Also identified by DOI 10.1039/b805774e.
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Abstract
A miniaturized flow injection analysis (FIA) system integrating a micropump on a microfluidic chip based on capillary and evaporation effects was developed. The pump was made by fixing a filter paper plug with a vent tube at the channel end, it requires no peripheral equipment and provides steady flow in the microl min(-1) range for FIA operation. Valve-free sample injection was achieved at nanolitre level using an array of slotted vials. The practical applicability of the system was demonstrated by DNA assay with laser-induced fluorescence (LIF) detection. A precision of 1.6% RSD (10.0 ng microl(-1), n=15) was achieved with a sampling throughput of 76 h(-1) and sample consumption of 95 nl.
Medical subject headings
- DNA
- Flow Injection Analysis
- Microfluidics