Cohesin subunit SMC1 associates with mitotic microtubules at the spindle pole.
basic_science · Level V
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- Record sourced from PubMed, PMID 18832153.
- Also identified by DOI 10.1073/pnas.0807660105 and PMC identifier 2557025.
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Abstract
Accurate mitotic chromosome segregation depends on the formation of a microtubule-based bipolar spindle apparatus. We report that the cohesin subunit structural maintenance of chromosomes subunit 1 (SMC1) is recruited to microtubule-bound RNA export factor 1 (Rae1) at the mitotic spindle pole. We locate the Rae1-binding site to a 21-residue-long region, SMC1(947-967) and provide several lines of evidence that phosphorylation of Ser(957) and Ser(966) of SMC1 stimulates binding to Rae1. Imbalances in these assembly pathways caused formation of multipolar spindles. Our data suggest that cohesin's known bundling function for chromatids in mitotic and interphase cells extends to microtubules at the spindle pole.
Medical subject headings
- Cell Cycle Proteins
- Chromosomal Proteins, Non-Histone
- Microtubules
- Spindle Apparatus