High-speed, miniaturized fluorescence microscopy in freely moving mice.

Flusberg, Benjamin A; Nimmerjahn, Axel; Cocker, Eric D; Mukamel, Eran A; Barretto, Robert P J; Ko, Tony H; Burns, Laurie D; Jung, Juergen C et al. · Nat Methods · 2008

basic_science · Level V

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Abstract

A central goal in biomedicine is to explain organismic behavior in terms of causal cellular processes. However, concurrent observation of mammalian behavior and underlying cellular dynamics has been a longstanding challenge. We describe a miniaturized (1.1 g mass) epifluorescence microscope for cellular-level brain imaging in freely moving mice, and its application to imaging microcirculation and neuronal Ca(2+) dynamics.

Medical subject headings