Efficient mapping of Applied Biosystems SOLiD sequence data to a reference genome for functional genomic applications.

Ondov, Brian D; Varadarajan, Anjana; Passalacqua, Karla D; Bergman, Nicholas H · Bioinformatics · 2008

basic_science · Level V

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Abstract

Here, we report the development of SOCS (short oligonucleotide color space), a program designed for efficient and flexible mapping of Applied Biosystems SOLiD sequence data onto a reference genome. SOCS performs its mapping within the context of 'color space', and it maximizes usable data by allowing a user-specified number of mismatches. Sequence census functions facilitate a variety of functional genomics applications, including transcriptome mapping and profiling, as well as ChIP-Seq. Executables, source code, and sample data are available at http://socs.biology.gatech.edu/

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