Kinetics of Wnt-driven beta-catenin stabilization revealed by quantitative and temporal imaging.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 18941539.
- Also identified by DOI 10.1371/journal.pone.0003498 and PMC identifier 2570213.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The Wnt/beta-catenin signal transduction pathway regulates a broad range of developmental processes. Aberrant activation of the Wnt pathway leads to cancer and degenerative diseases. beta-catenin is a key signaling molecule that is frequently used as a direct monitor of Wnt pathway activation. This paper describes a multi-parametric method for quantitative analysis of cellular beta-catenin protein levels in a rapid and high-throughput manner. The assay offers temporally resolved detection of Wnt-stimulated accumulation of beta-catenin, simultaneously detecting cell number, and it sheds light onto the kinetics of posttranslational stabilization of beta-catenin.
Medical subject headings
- Proteomics
- Wnt Proteins
- beta Catenin