A noncytotoxic DsRed variant for whole-cell labeling.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 18953349.
- Also identified by DOI 10.1038/nmeth.1264 and PMC identifier 4107390.
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Abstract
A common application of fluorescent proteins is to label whole cells, but many RFPs are cytotoxic when used with standard high-level expression systems. We engineered a rapidly maturing tetrameric fluorescent protein called DsRed-Express2 that has minimal cytotoxicity. DsRed-Express2 exhibits strong and stable expression in bacterial and mammalian cells, and it outperforms other available RFPs with regard to photostability and phototoxicity.
Medical subject headings
- Luminescent Proteins
- Staining and Labeling