Nanoscale imaging of molecular positions and anisotropies.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19011626.
- Also identified by DOI 10.1038/nmeth.1271 and PMC identifier 2901392.
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Abstract
Knowledge of the orientation of molecules within biological structures is crucial to understanding the mechanisms of cell function. We present a method to image simultaneously the positions and fluorescence anisotropies of large numbers of single molecules with nanometer lateral resolution within a sample. Based on a simple modification of fluorescence photoactivation localization microscopy (FPALM), polarization (P)-FPALM does not compromise speed or sensitivity. We show results for mouse fibroblasts expressing Dendra2-actin or Dendra2-hemagglutinin.
Medical subject headings
- Biopolymers
- Fibroblasts
- Microscopy, Fluorescence
- Nanostructures
- Nanotechnology