A large genome center's improvements to the Illumina sequencing system.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19034268.
- Also identified by DOI 10.1038/nmeth.1270 and PMC identifier 2610436.
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Abstract
The Wellcome Trust Sanger Institute is one of the world's largest genome centers, and a substantial amount of our sequencing is performed with 'next-generation' massively parallel sequencing technologies: in June 2008 the quantity of purity-filtered sequence data generated by our Genome Analyzer (Illumina) platforms reached 1 terabase, and our average weekly Illumina production output is currently 64 gigabases. Here we describe a set of improvements we have made to the standard Illumina protocols to make the library preparation more reliable in a high-throughput environment, to reduce bias, tighten insert size distribution and reliably obtain high yields of data.
Medical subject headings
- Academies and Institutes
- Chromosome Mapping
- Genomics
- Polymerase Chain Reaction
- Sequence Analysis, DNA