Microscopes for fluorimeters: the era of single molecule measurements.

Vale, Ronald D · Cell · 2008

review · Level V

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Abstract

As transforming as the first atomic resolution view of myoglobin in the late 1950s, scientists can now use a suite of single molecule technologies to watch protein macromolecular machines executing their functions "in real time." This Essay highlights applications and challenges of single molecule studies in structural biology, cell biology, and biotechnology.

Medical subject headings