High affinity scFv-hapten pair as a tool for quantum dot labeling and tracking of single proteins in live cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19053789.
- Also identified by DOI 10.1021/nl8032284 and PMC identifier 3084663.
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Abstract
We describe a general approach to label cell surface proteins using quantum dots (QD) for single-molecule tracking. QDs coated with small-hapten modified peptides are targeted to cell surface fusion proteins containing the corresponding single-chain fragment antibody (scFv). The approach is illustrated with the small hapten fluorescein (FL) and a high-affinity anti-FL scFv fused to two different proteins in yeast and murine neuronal cell line N2a.
Medical subject headings
- Haptens
- Immunoglobulin Fragments
- Proteins
- Quantum Dots