One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome.
basic_science · Level V
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- Record sourced from PubMed, PMID 19073939.
- Also identified by DOI 10.1073/pnas.0811011106 and PMC identifier 2600582.
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Abstract
We previously reported assembly and cloning of the synthetic Mycoplasma genitalium JCVI-1.0 genome in the yeast Saccharomyces cerevisiae by recombination of six overlapping DNA fragments to produce a 592-kb circle. Here we extend this approach by demonstrating assembly of the synthetic genome from 25 overlapping fragments in a single step. The use of yeast recombination greatly simplifies the assembly of large DNA molecules from both synthetic and natural fragments.
Medical subject headings
- DNA
- Genome, Bacterial
- Mycoplasma genitalium
- Oligodeoxyribonucleotides
- Yeasts