Infrared laser-mediated gene induction in targeted single cells in vivo.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19079252.
- Also identified by DOI 10.1038/nmeth.1278.
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Abstract
We developed infrared laser-evoked gene operator (IR-LEGO), a microscope system optimized for heating cells without photochemical damage. Infrared irradiation causes reproducible temperature shifts of the in vitro microenvironment in a power-dependent manner. When applied to living Caenorhabditis elegans, IR-LEGO induced heat shock-mediated expression of transgenes in targeted single cells in a more efficient and less deleterious manner than a 440-nm dye laser and elicited physiologically relevant phenotypic responses.
Medical subject headings
- Caenorhabditis elegans
- Caenorhabditis elegans Proteins
- Gene Expression Regulation
- Lasers
- Microscopy, Confocal