Sensitive, specific polymorphism discovery in bacteria using massively parallel sequencing.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19079253.
- Also identified by DOI 10.1038/nmeth.1286 and PMC identifier 2613166.
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Abstract
Our variant ascertainment algorithm, VAAL, uses massively parallel DNA sequence data to identify differences between bacterial genomes with high sensitivity and specificity. VAAL detected approximately 98% of differences (including large insertion-deletions) between pairs of strains from three species while calling no false positives. VAAL also pinpointed a single mutation between Vibrio cholerae genomes, identifying an antibiotic's site of action by identifying sequence differences between drug-sensitive strains and drug-resistant derivatives.
Medical subject headings
- DNA, Bacterial
- Polymorphism, Genetic