An in vitro microfluidic approach to generating protein-interaction networks.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19098921.
- Also identified by DOI 10.1038/nmeth.1289 and PMC identifier 4117197.
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Abstract
We developed an in vitro protein expression and interaction analysis platform based on a highly parallel and sensitive microfluidic affinity assay, and used it for 14,792 on-chip experiments, which exhaustively measured the protein-protein interactions of 43 Streptococcus pneumoniae proteins in quadruplicate. The resulting network of 157 interactions was denser than expected based on known networks. Analysis of the network revealed previously undescribed physical interactions among members of some biochemical pathways.
Medical subject headings
- Bacterial Proteins
- Microfluidic Analytical Techniques