Label-free determination of protein-surface interaction kinetics by ionic conductance inside a nanochannel.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19107291.
- Also identified by DOI 10.1039/b811006a.
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Abstract
We have developed a label-free conductometric platform for the rapid measurement of kinetics parameters for the adsorption and desorption of proteins on surfaces. Adsorbed Bovine Serum Albumin (BSA) has been detected electrically with response times in the minute range, and kinetic models were elaborated and compared to the measurements. The device presents similar characteristics to Surface Plasmon Resonance (SPR) immunosensors, but takes advantage of a simpler, low-cost electronic measurement unit.
Medical subject headings
- Biosensing Techniques
- Proteins