Genetically timed, activity-sensor and rainbow transsynaptic viral tools.
basic_science · Level V
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- Record sourced from PubMed, PMID 19122667.
- Also identified by DOI 10.1038/nmeth.1292.
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Abstract
We developed retrograde, transsynaptic pseudorabies viruses (PRVs) with genetically encoded activity sensors that optically report the activity of connected neurons among spatially intermingled neurons in the brain. Next we engineered PRVs to express two differentially colored fluorescent proteins in a time-shifted manner to define a time period early after infection to investigate neural activity. Finally we used multiple-colored PRVs to differentiate and dissect the complex architecture of brain regions.
Medical subject headings
- Green Fluorescent Proteins
- Herpesvirus 1, Suid
- Luminescent Proteins
- Synaptic Transmission
- Visual Pathways