miRNA in situ hybridization in formaldehyde and EDC-fixed tissues.
other · Level V
Where this comes from
- Record sourced from PubMed, PMID 19137005.
- Also identified by DOI 10.1038/nmeth.1294 and PMC identifier 2838186.
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Abstract
MicroRNAs are small regulatory RNAs with many biological functions and disease associations. We showed that in situ hybridization (ISH) using conventional formaldehyde fixation results in substantial microRNA loss from mouse tissue sections, which can be prevented by fixation with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide that irreversibly immobilizes the microRNA at its 5' phosphate. We determined optimal hybridization parameters for 130 locked nucleic acid probes by recording nucleic acid melting temperature during ISH.
Medical subject headings
- Carbodiimides
- Formaldehyde
- In Situ Hybridization
- MicroRNAs
- Tissue Fixation