Transgenic mice with defined combinations of drug-inducible reprogramming factors.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19151700.
- Also identified by DOI 10.1038/nbt.1520 and PMC identifier 2654270.
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Abstract
Proviruses carrying drug-inducible Oct4, Sox2, Klf4 and c-Myc used to derive 'primary' induced pluripotent stem (iPS) cells were segregated through germline transmission, generating mice and cells carrying subsets of the reprogramming factors. Drug treatment produced 'secondary' iPS cells only when the missing factor was introduced. This approach creates a defined system for studying reprogramming mechanisms and allows screening of genetically homogeneous cells for compounds that can replace any transcription factor required for iPS cell derivation.
Medical subject headings
- Cell Differentiation
- Doxycycline
- Genetic Techniques
- Transcription Factors