In vivo imaging of extracellular matrix remodeling by tumor-associated fibroblasts.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19151720.
- Also identified by DOI 10.1038/nmeth.1295 and PMC identifier 2877598.
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Abstract
Here we integrated multiphoton laser scanning microscopy and the registration of second harmonic generation images of collagen fibers to overcome difficulties in tracking stromal cell-matrix interactions for several days in live mice. We show that the matrix-modifying hormone relaxin increased tumor-associated fibroblast (TAF) interaction with collagen fibers by stimulating beta1-integrin activity, which is necessary for fiber remodeling by matrix metalloproteinases.
Medical subject headings
- Collagen
- Extracellular Matrix
- Microscopy, Confocal
- Soft Tissue Neoplasms