Large-scale profiling of archival lymph nodes reveals pervasive remodeling of the follicular lymphoma methylome.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19155300.
- Also identified by DOI 10.1158/0008-5472.CAN-08-2984 and PMC identifier 7213763.
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Abstract
Emerging technologies allow broad profiling of the cancer genome for differential DNA methylation relative to benign cells. Herein, bisulfite-modified DNA from lymph nodes with either reactive hyperplasia or follicular lymphoma (FL) were analyzed using a commercial C/UpG genotyping assay. Two hundred fifty-nine differentially methylated targets (DMT) distributed among 183 unique genes were identified in FL. Comparison of matched formalin-fixed, paraffin-embedded and frozen surgical pathology replicates showed the complete preservation of the cancer methylome among differently archived tissue specimens. Analysis of the DMT profile is consistent with a pervasive epigenomic remodeling process in FL that affects predominantly nonlymphoid genes.
Medical subject headings
- Lymph Nodes
- Lymphoma, Follicular