Absolute brightness of fluorescent microspheres.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19156300.
- Also identified by DOI 10.1039/b810219h.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
The absolute brightness of fluorescent particles, such as dye-containing nano- and microspheres or quantum dots, is a critical design parameter for many applications relying on fluorescence detection. The absolute brightness, defined as the ratio of radiant intensity of emission to illumination intensity of excitation, of nile-red fluorescent microspheres with a 1 micrometre diameter is measured to be 4.2 +/- 1 x 10(-16) m(2)/sr, and the implications for the design of kinesin motor protein-powered "smart dust" devices and the remote detection of fluorescence are discussed.
Medical subject headings
- Fluorescence
- Microscopy, Fluorescence
- Microspheres