Efficient microRNA capture and bar-coding via enzymatic oligonucleotide adenylation.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19160512.
- Also identified by DOI 10.1038/nmeth.1244.
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Abstract
Here we report a highly efficient and simplified strategy to preadenylate bar-coded oligonucleotides designed for microRNA (miRNA) capture and multiplex analysis. Using this approach, we enzymatically preadenylated bar-coded oligonucleotides with high efficiency when compared to the chemical method currently used by miRNA investigators. As a case study, we used these oligonucleotides in an ATP-independent ligation to miRNAs, suggesting the utility of our method in end-capture protocols and high-throughput sequencing applications.
Medical subject headings
- Adenine
- DNA Ligases
- Electronic Data Processing
- MicroRNAs
- Oligonucleotides
- RNA Ligase (ATP)