A bright and photostable photoconvertible fluorescent protein.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19169260.
- Also identified by DOI 10.1038/nmeth.1296 and PMC identifier 2745648.
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Abstract
Photoconvertible fluorescent proteins are potential tools for investigating dynamic processes in living cells and for emerging super-resolution microscopy techniques. Unfortunately, most probes in this class are hampered by oligomerization, small photon budgets or poor photostability. Here we report an EosFP variant that functions well in a broad range of protein fusions for dynamic investigations, exhibits high photostability and preserves the approximately 10-nm localization precision of its parent.
Medical subject headings
- Fluorescent Dyes
- Luminescent Proteins