Screening a peptide library by DSC and SAXD: comparison with the biological function of the parent proteins.
other · Level V
Where this comes from
- Record sourced from PubMed, PMID 19194494.
- Also identified by DOI 10.1371/journal.pone.0004356 and PMC identifier 2632743.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We have recently identified the membranotropic regions of the hepatitis C virus proteins E1, E2, core and p7 proteins by observing the effect of protein-derived peptide libraries on model membrane integrity. We have studied in this work the ability of selected sequences of these proteins to modulate the L(beta)-L(alpha) and L(alpha)-H(II) phospholipid phase transitions as well as check the viability of using both DSC and SAXD to screen a protein-derived peptide library. We demonstrate that it is feasible to screen a library of peptides corresponding to one or several proteins by both SAXD and DSC. This methodological combination should allow the identification of essential regions of membrane-interacting proteins which might be implicated in the molecular mechanism of membrane fusion and/or budding.
Medical subject headings
- Peptide Library
- Scattering, Small Angle
- Viral Proteins
- X-Ray Diffraction