Direct determination of haplotypes from single DNA molecules.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19198595.
- Also identified by DOI 10.1038/nmeth.1301 and PMC identifier 3880790.
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Abstract
Determining the long-range haplotypes in a diploid individual is a major technical challenge. Here we report a method of molecular haplotyping by directly imaging multiple polymorphic sites on individual human DNA molecules simultaneously. We demonstrate the utility of this technology by accurately determining the haplotypes consisting of up to 16 single-nucleotide polymorphisms in genomic regions up to 50 kilobases.
Medical subject headings
- Haplotypes
- Polymorphism, Single Nucleotide
- Sequence Analysis, DNA