Sensing of UO22+ and design of logic gates by the application of supramolecular constructs of ion-dependent DNAzymes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19199475.
- Also identified by DOI 10.1021/nl803887y.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Supramolecular constructs composed of ion-dependent DNAzymes and their substrates were used to develop DNAzyme cascades that enabled the sensitive detection of UO22+ or the activation of logic gate operations. The supramolecular complex between the UO22+-dependent DNAzyme and its substrate leads, in the presence of UO22+, to the cleavage of the substrate and to the release of the HRP-mimicking DNAzyme that enables the optical analysis of UO22+ (detection limit 1 x 10-9 M). Similarly, supramolecular complexes between the Mg2+- and UO22+-dependent DNAzymes and tailored substrates enables the design of the "OR" and "AND" logic gates, using Mg2+ and UO22+ as inputs.
Medical subject headings
- DNA
- DNA, Catalytic