Picomolar detection of protease using peptide/single walled carbon nanotube/gold nanoparticle-modified electrode.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19206503.
- Also identified by DOI 10.1021/nn8000774.
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Abstract
Picomolar electrochemical detection of human immunodeficiency virus type-1 protease (HIV-1 PR) using ferrocene (Fc)-pepstatin-modified surfaces has been presented. Gold electrode surface was modified with gold nanoparticles (AuNP) or thiolated single walled carbon nanotubes/gold nanoparticles (SWCNT/AuNP). Thiol-terminated Fc-pepstatin was then self-assembled on such surfaces as confirmed by Raman spectroscopy and scanning electron microscope. The interaction between the Fc-pepstatin-modified substrates and HIV-1 PR was studied by cyclic voltammetry and electrochemical impedance spectroscopy. Both electrode materials showed enhanced electrochemical responses to increasing concentrations of HIV-1 PR with shifting to higher potentials as well as decrease in the overall signal intensity. However, the sensing electrode modified with thiolated SWCNTs/AuNPs showed remarkable detection sensitivity with an estimated detection limit of 0.8 pM.
Medical subject headings
- Biosensing Techniques
- Electrochemistry
- Gold
- HIV Protease
- Microelectrodes
- Nanotechnology
- Nanotubes, Carbon