Computer-enhanced high-throughput genetic screens of C. elegans in a microfluidic system.

Crane, Matthew M; Chung, Kwanghun; Lu, Hang · Lab Chip · 2009

basic_science · Level V

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Abstract

Most visual screens based on fluorescent markers are currently limited in throughput and accuracy. Here we present the first genetic screen of a mutagenized population of C. elegans in a microfluidic device. Animal handling streamlined by a microfluidic device and intuitive control software enabled the identification of novel mutants and a large screening speed.

Medical subject headings