Dynamic cell culture: a microfluidic function generator for live cell microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 19209350.
- Also identified by DOI 10.1039/b807682k.
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Abstract
We present a microfluidic system for time-lapsed, live cell microscopy with the ability to control solution exchange via a dynamic flow controller. The application specific microfluidic plates are designed to maintain adherent and non-adherent cell types for multiple days with continuous medium perfusion. Upstream channels with flow controlled via custom software allow the delivery of unique exposure profiles to the cultured cells, such as square waves, step functions, ramps, etc.
Medical subject headings
- Microfluidics
- Microscopy