Fabrication of permeable tubular constructs from chemically modified chitosan with enhanced antithrombogenic property.

Abstract

The failure of artificial vascular grafts in small diameter vessel replacement is mainly due to the early formation of thrombosis. To prevent the occurrence of thrombosis, much effort has been focused on developing an anti-thrombogenic coating of synthetic vascular prostheses or artificial conduits with improved anti-thrombogenic properties. Because surface coatings may be unstable for long-term applications, a bulk material with anti-thrombogenic property is desirable for the fabrication of vascular grafts or conduits. To this end, we have chemically modified chitosan by phthalization to derive an anti-thrombogenic material for the fabrication of vascular grafts. The chemical structure of phthalized chitosan was characterized with infrared spectroscopy. The hydrophilicity was examined with contact angle measurement, and the molecular weight distribution was measured using gel permeation chromatography (GPC). Protein adsorption, hemolysis, and platelet adhesion assays were used to confirm the enhanced anti-thrombogenic properties of this phthalized chitosan. Cytotoxicity and proliferation assays were performed to test its high biocompatibility. With its improved solubility and processibility, this phthalized chitosan was fabricated into selective permeable tubular constructs of varying sizes and morphology through a wet phase-inversion process. With improved anti-thrombogenic property, biocompatibility, and great processibility, phthalized chitosan has great potential as the material for the fabrication of small diameter vascular grafts.